蛋白
抗体
检测试剂盒
蛋白MARKER
分子生物学
规格：
查看说明书| Product Name: | RAG2 Rabbit pAb |
| Cat No.: | DPA03574 |
| Clonality: | Polyclonal |
| Species Reactivity: | Mouse |
| Tested Applications: | WB |
| Recommended Dilution: | WB: 1:1000-1:5000 |
| Size: | 30ul 50ul 100uL |
| Format: | Liquid |
| Source: | Rabbit |
| Purification Method: | Affinity Purification |
| Isotype: | IgG |
| Conjugate: | Un-conjugated |
| Storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide a |
| Immunogen: | A synthetic peptide of mouse RAG2 |
| Calculated Molecular Weight: | 59 kDa |
| Observed Molecular Weight: | 59 kDa |
| GenBank Accession Number: | P21784 |
| Gene ID (NCBI): | 19374 |
| Synonyms: | Rag-2 |
| Background: | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. |
| Category: | Primary Ab |
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