蛋白
抗体
检测试剂盒
蛋白MARKER
分子生物学
规格：
查看说明书PHD3 Rabbit pAb
Cat#:DPA03286应用：WB,IHC,ICC/IF,IP
| Product Name: | PHD3 Rabbit pAb |
| Cat No.: | DPA03286 |
| Clonality: | Polyclonal |
| Species Reactivity: | Human, Mouse, Rat |
| Tested Applications: | WB,IHC,ICC/IF,IP |
| Recommended Dilution: | WB: 1:1000 IHC: 1:50 ICC/IF: 1:50 IP: 1:20 |
| Size: | 30ul 50ul 100uL |
| Format: | Liquid |
| Source: | Rabbit |
| Purification Method: | Affinity Purification |
| Isotype: | IgG |
| Conjugate: | Un-conjugated |
| Storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide a |
| Immunogen: | Recombinant protein of human PHD3 |
| Calculated Molecular Weight: | 27 kDa |
| Observed Molecular Weight: | 27 kDa |
| GenBank Accession Number: | Q9H6Z9 |
| Gene ID (NCBI): | 112399 |
| Synonyms: | PHD3; HIFPH3; HIFP4H3 |
| Background: | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. |
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